A double blind study was designed to determine the effects of folate mouthwash on established gingivitis in non-pregnant adults. 60 subjects who had greater than 20 teeth, visible gingival inflammation around greater than 6 teeth, no complicated medical history, currently not receiving periodontal treatment or medication, and not wearing dentures, were randomly assigned to control or experimental groups. Full mouth assessment included plaque scores, gingival colour changes, bleeding tendency around every tooth and experience of disease and local factors.
Subjects used 5 ml of mouthwasg twice daily for 4 weeks, rinsing for 1 min before expectorating. Experimental mouthwash contained 5 mg folate per 5 ml. The control group used a placebo mouthwash. A detailed 3-day diet record was kept by each subject. The oral examination was repeated after 4 weeks. Initially, groups were similar except that the experimental group exhibited more bleeding sites at the outset, but after 4 weeks, the experimental group showed a significant decrease in mean number of colour change sites and in bleeding sites compared with control group.
According to the researchers:
“Folate mouthwash appears to have an influence on gingival health through local rather than systemic influence.”